The condensed protocols from Molecular cloning : a laboratory manual /
| Main Author: | |
|---|---|
| Corporate Author: | |
| Other Authors: | |
| Format: | Book |
| Language: | English |
| Published: |
Cold Spring Harbor, N.Y.:
Cold Spring Harbor Laboratory Press,
c2006
|
| Subjects: |
Table of Contents:
- Plasmids and their usefulness in molecular cloning
- Bacteriophage and its vectors
- Working with bacteriophage M13 vectors
- Working with high-capacity vectors
- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
- Preparation and analysis of eukaryotic genomic DNA
- Extraction, purification, and analysis of mRNA from eukaryotic cells
- In vitro amplification of DNA by the polymerase chain reaction
- Preparation of radiolabeled DNA and RNA probes
- Working with synthetic oligonucleotide probes
- Preparation of cDNA libraries and gene identification
- DNA sequencing
- Mutagenesis
- Screening expression libraries
- Expression of cloned genes in escherichia coli
- Introducing cloned genes into cultured mammalian cells
- Analysis of gene expression in cultured mammalian cells
- Protein interaction technologies