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02053nam a2200253 a 4500 |
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20171112000054.0 |
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090320s2006 gr r 000 0 eng d |
| 020 |
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|a 0879697725
|q σκληρόδετο
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| 020 |
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|a 0879697717
|q χαρτόδ.
|z 9780879697716
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| 040 |
|
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|a GR-KoDPT
|b gre
|e AACR2
|
| 050 |
|
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|a QH442.2
|b .S24 2006
|
| 100 |
1 |
|
|a Sambrook, Joseph
|
| 245 |
1 |
4 |
|a The condensed protocols from Molecular cloning :
|b a laboratory manual /
|c Joseph Sambrook, David W. Russell
|
| 260 |
1 |
4 |
|a Cold Spring Harbor, N.Y.:
|b Cold Spring Harbor Laboratory Press,
|c c2006
|
| 300 |
1 |
4 |
|a v, 800 σ. :
|b εικ. ;
|c 28 εκ.
|
| 500 |
1 |
4 |
|a "A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--σ. [4] του εξωφύλλου
|
| 504 |
1 |
4 |
|a Περιέχει βιβλιογραφικές παραπομπές και ευρετήριο
|
| 505 |
0 |
4 |
|a Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies
|
| 650 |
0 |
0 |
|a Molecular cloning
|x Laboratory manuals
|
| 700 |
1 |
0 |
|a Russell, David W.
|
| 710 |
1 |
0 |
|a Cold Spring Harbor Laboratory
|
| 952 |
|
|
|a GRHeTEI
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