Evolutionary methods in biotechnology : clever tricks for directed evolution /
Other Authors: | , |
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Format: | Book |
Language: | English |
Published: |
Weinheim:
Wiley-VCH,
c2004
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Subjects: |
Table of Contents:
- 1. Introduction
- 2. Generation of mutant libraries using random mutagenesis
- 3. DNA shuffling
- 4. DNA recombination using StEP
- 5. FACS screening of combinatorial peptide and protein libraries displayed on the surface of escherichia coli cells
- 6. Selection of phage-displayed enzymes
- 7. Selection of aptamers
- 8. Methods for selecting catalytic nucleic acids
- 9. High-throughput screening of enantioselective industrial biocatalysts
- 10. Computer-assisted design of doped libraries
- 11. Directed in silico mutagenesis
- 12. RNA folding in silico
- 13. Patenting in evolutionary biotechnology
- Subject index