Evolutionary methods in biotechnology : clever tricks for directed evolution /

Other Authors: Brakmann, Susanne, Schwienhorst, Andreas
Format: Book
Language:English
Published: Weinheim: Wiley-VCH, c2004
Subjects:
Table of Contents:
  • 1. Introduction
  • 2. Generation of mutant libraries using random mutagenesis
  • 3. DNA shuffling
  • 4. DNA recombination using StEP
  • 5. FACS screening of combinatorial peptide and protein libraries displayed on the surface of escherichia coli cells
  • 6. Selection of phage-displayed enzymes
  • 7. Selection of aptamers
  • 8. Methods for selecting catalytic nucleic acids
  • 9. High-throughput screening of enantioselective industrial biocatalysts
  • 10. Computer-assisted design of doped libraries
  • 11. Directed in silico mutagenesis
  • 12. RNA folding in silico
  • 13. Patenting in evolutionary biotechnology
  • Subject index