Electrophoresis in practice : a guide to methods and applications of DNA and protein separations /

Main Author: Westermeier, Reiner.
Other Authors: Gronau, Sonja.
Format: Book
Language:English
German
Published: Weinheim : Wiley-VCH, c2005.
Edition:4th rev. and enl. ed.
Subjects:
Table of Contents:
  • Electrophoresis.
  • Isotachophoresis.
  • Isoelectric focusing.
  • Blotting.
  • Interpretation of electropherograms.
  • Proteome analysis.
  • Instrumentation.
  • Equipment and methods: PAGE of dyes ; Agarose and immuno electrophoresis ; Titration curve analysis ; Native PAGE in amphoteric buffers ; Agarose IEF ; PAGIEF in rehydrated gels ; Horizontal SDS-PAGE ; Vertical PAGE ; Semi-dry blotting of proteins ; IEF in immobilized pH gradients ; High-resolution 2-D electrophoresis ; PAGE of double stranded DNA ; Native PAGE of single stranded DNA ; Denaturing gradient gel electrophoresis ; Denaturing PAGE of DNA.
  • Appendix: Trouble-shooting.