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| LEADER |
03612nam a2200301 a 4500 |
| 001 |
1980938 |
| 005 |
20171111235916.0 |
| 008 |
071001s1999 cau o 001 0 eng d |
| 020 |
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|a 9780080536828
|q (electronic bk.)
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| 020 |
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|a 0080536824
|q (electronic bk.)
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| 020 |
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|a 9780125839907
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| 020 |
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|a 0125839901
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| 040 |
|
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|a N$T
|b eng
|e pn
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| 050 |
|
4 |
|a QH506
|b .R436 1999eb
|
| 100 |
1 |
4 |
|a Ream, Walt.
|
| 245 |
1 |
0 |
|a Molecular biology techniques :
|b an intensive laboratory course /
|c Walt Ream and Katharine G. Field.
|
| 260 |
1 |
0 |
|a San Diego ;
|b Academic Press,
|c 1999.
|a London :
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| 300 |
1 |
0 |
|a 1 online resource (x, 234 pages)
|
| 505 |
0 |
0 |
|a Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index.
|
| 650 |
0 |
0 |
|a Molecular biology
|x Technique.
|
| 650 |
0 |
2 |
|a Molecular Biology.
|
| 650 |
0 |
4 |
|a Moleküler biyoloji
|x Laboratuvar el kitapları
|
| 650 |
0 |
6 |
|a Biologie moléculaire
|
| 650 |
0 |
7 |
|a SCIENCE
|x Life Sciences
|x Molecular Biology.
|
| 650 |
0 |
7 |
|a Molecular biology
|x Technique.
|
| 700 |
1 |
7 |
|a Field, Katharine G.
|
| 952 |
|
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|a GrAtEKP
|b 59cd1ef86c5ad13446103b09
|c 998a
|d 945l
|e 572.8 ReaW m 1999
|t 1
|x m
|z Books
|
| 952 |
|
|
|a CY-NiOUC
|b 5a04670a6c5ad14ac1ef009d
|c 998a
|d 945l
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|z Books
|
