RNA methodologies : a laboratory guide for isolation and characterization /
| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
Amsterdam ; Boston :
Elsevier/Academic Press,
©2005.
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| Edition: | 3rd ed. |
| Subjects: | |
| Online Access: | http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=166200 |
Table of Contents:
- RNA and the cellular biochemistry revisited
- Transcription and the organization of eukaryotic genes
- Messenger RNA
- Resilient ribonucleases
- RNA isolation strategies
- The truth about tissues
- Isolation of polyadenylated RNA
- Quality control for RNA preparations
- Dot blot analysis
- Electrophoresis of RNA
- Photodocumentation and image analysis
- Northern analysis
- Nucleic acid probe technology
- Practical nucleic acid hybridization
- Principles of detection
- Quantification of specific mRNAs by nuclease protection
- Analysis of nuclear RNA
- cDNA synthesis
- RT-PCR
- Quantitative PCR techniques
- Transcript subtraction methods
- mRNA differential display
- High-throughput analysis of gene expression
- RNA interference : targeted gene silencing
- Genomes, transcriptomes, proteomes, and bioinformatics
- An RNA Paradigm
- Epilogue : a few pearls of wisdom.