Environmental microbiology : a laboratory manual /

Main Author: Pepper, Ian L.
Other Authors: Gerba, Charles P.,
Format: Book
Language:English
Published: Amsterdam ; Boston : Elsevier Academic Press, ©2005.
Edition:2nd ed.
Subjects:
Online Access:http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=187198
Table of Contents:
  • Cover
  • Table of Contents
  • Preface
  • BASICS
  • MANUAL CONVENTIONS
  • SUGGESTED SOIL TYPES AND TESTS
  • SECTION ONE: Basic Protocols
  • EXPERIMENT 1: Dilution and Plating of Bacteria and Growth Curves
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • EXAMPLE CALCULATION OF MEAN GENERATION TIME
  • QUESTIONS AND PROBLEMS
  • REFERENCE
  • EXPERIMENT 2: Soil Moisture Content Determination
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • EXAMPLE CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • SECTION TWO: Examination of Soil Microorganisms Via Microscopic and Cultural Assays
  • EXPERIMENT 3: Contact Slide Assay
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 4: Filamentous Fungi
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 5: Bacteria and Actinomycetes
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 6: Algae: Enumeration by MPN
  • OVERVIEW
  • THEORY
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • SECTION THREE: Microbial Transformations and Response to Contaminants
  • EXPERIMENT 7: Oxidation of Sulfur in Soil
  • OVERVIEW
  • THEORY
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 8: Dehydrogenase Activity of Soils
  • OVERVIEW
  • THEORY
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • EXAMPLE CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 9: Nitrification and Denitrification
  • OVERVIEW
  • THEORY
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • ASSIGNMENT AND QUESTIONS
  • REFERENCES
  • EXPERIMENT 10: Enrichment and Isolation of Bacteria that Degrade 2,4-Dichlorophenoxyacetic Acid
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 11: Adaptation of Soil Bacteria to Metals
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTIAL HAZARDS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 12: Biodegradation of Phenol Compounds
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • POTENTIAL HAZARDS
  • CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 13: Assimilable Organic Carbon
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • CALCULATIONS
  • QUESTIONS AND PROBLEMS
  • REFERENCES
  • EXPERIMENT 14: Biochemical Oxygen Demand
  • OVERVIEW
  • THEORY AND SIGNIFICANCE
  • PROCEDURE
  • TRICKS OF THE TRADE
  • POTENTI.