Protein phosphatase protocols/

Other Authors: Moorhead, Greg
Format: Book
Language:English
Published: Totowa, N.J.: Humana Press, c2007
Series:Methods in molecular biology, 365
Subjects:
Online Access:http://www.loc.gov/catdir/toc/ecip069/2006007128.html
http://www.loc.gov/catdir/enhancements/fy0825/2006007128-d.html
Table of Contents:
  • Analysis of protein phosphatases / Shirish Shenolikar
  • A brief introduction to the protein phosphatase families / Tomas Mustelin
  • Small molecule inhibitors of Ser/thr protein phosphatases / Mark Swingle, Li Ni, and Richard E. Honkanen
  • Synthesis and use of the protein phosphatase affinity matrices microcystin- and microcystin-biotin-sepharose / Greg Moorhead, Timothy A.J. Haystead, and Carol MacKintosh
  • Utilizing protein phosphatase inhibitors to define PP2A as a regulator of ataxia-telangiectasia mutated (ATM) / Aaron A. Goodarzi .. [et al.]
  • An automated, fluorescence-based method for continuous assay of PP2A activity / Adam M. Wegner .. [et al.]
  • An in vivo assay to quantify stable protein phosphatase 2A (PP2A) heterotrimeric species / Matthew S. Gentry, Richard L. Hallberg, and David C. Pallas
  • Mutagenesis and expression of the scaffolding Aa and Ab subunits of PP2A / Ralf Ruediger, Jin Zhou, and Gernot Walter
  • Isolation and characterization of PP2A holoenzymes containing FLAG-tagged B subunits / Deanna G. Adams and Brian E. Wadzinski
  • Purification of PP2A holoenzymes by sequential immunoprecipitation with anti-peptide antibodies / Gernot Walter, Jin Zhou, and Ralf Ruediger
  • Purification of PP2Ac from bovine heart / Hue T. Tran .. [et al.]
  • Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions / Laura Trinkle-Mulcahy .. [et al.]
  • Yeast two-hybrid screens to identify drosophila PP1-binding proteins / Daimark Bennett and Luke Alphey
  • Identification of cellular protein phosphatase-1 regulators / David W. Roadcap, Matthew H. Brush, and Shirish Shenolikar